محیط کشت BHI Borth

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Microbial penetration
Scaffolds were placed on open 10 ml vials containing 5ml of Brain heart infusion (BHI) broth
(Merck, Germany) (test area: 0.8 cm2) to assess their ability to prevent microbial invasion
Bottles covered with the cotton ball and open vials used as negative and positive contro
respectively. The tested vials were placed in an open environment for 3 and 7days. The
cloudiness of the nutrient broth in any vial was recorded as microbial contamination.
Spectrophotometric measurements were obtained at 600 nm in a microplate spectrophotometer
(n=3).